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Most of his songs are written in partnership with his friend, singer and songwriter Erasmo Carlos. The family lived in a modest home on top of a hill in the Nook neighbourhood. At age 6, during the feast of St Peter, the patron saint of Cachoeiro do Itapemirim, Roberto Carlos was hit by a steam locomotive and had to have his right leg amputated just below his knee. As a child he learned to play the guitar and the piano -— first with his mother and later at the Itapemirim Music Conservatory.

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This cannot currently be explained by the usual environmental or by host genetic factors. Preliminary studies by Pei and colleagues at NYU on elderly male veterans identified two types of microbiotas in the esophagus. In a small scale study, we also found that 3 of 3 cases of EA harbored the type II biota. The findings have opened a new approach to understanding the recent surge in the incidence of EA.

Our long-term goal is to identify the cause of GERD sequence. We will conduct a case control study to demonstrate the microbiome disease association in every stage of GERD sequence, as well as analyze the trend in changes in the microbiome along disease progression toward EA, by two specific aims. Aim 1 is to conduct a comprehensive population survey of the foregut microbiome and demonstrate its association with GERD sequence. Furthermore, spatial relationship between the esophageal microbiota and upstream mouth and downstream stomach foregut microbiotas as well as temporal stability of the microbiome-disease association will also be examined.

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